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In Vitro Activation of Dormant Follicles for Patients With Primary Ovarian Insufficiency
Not Recruiting
Trial ID: NCT02322060
Purpose
In this study, the investigators used the newly developed technique i.e. in vitro activation
of dormant follicles (IVA) to promote ovarian follicle growth much more efficiently than
natural, in vivo process for women with Primary Ovarian Insufficiency (POI).Firstly, the
investigators remove one ovary under laparoscopic surgery. Then, we dissect ovarian cortex
from the ovarian medulla. The ovarian cortex is cut into small cubes and cultured with medium
containing drugs to activate dormant follicles. After 2 days of culture, the ovarian cubes
are transplanted mainly beneath the membrane of Fallopian tubes under laparoscopic surgery.
The ovarian cortex could be cryopreserve for future re-transplantation and in some cases, for
convenience to arrange second surgery. Once frozen, the ovary can be preserved
semipermanently. After transplantation, patients receive ultrasound monitoring together with
measurement of serum hormone levels for 10-12 months. If growing follicles are detected,
follicle growth is stimulated by injection of hormones (gonadotropins). Using the same "ovum
pick up" approach used in IVF (in vitro fertilization), we pick up oocytes from the follicles
and fertilize them. Fertilized eggs are cultured and then cryopreserved for future embryo
transfer.
Currently, we recurit patients diagnosed with POI, or Ovarian resistance syndrome (ORS). The
procedure can also be: Only superficial cut of the ovarian cortex by laparoscopy or
laparotomy, without taking ovary outside or cultured with medium.
Official Title
Ovarian Tissue Cryopreservation and Auto-transplantation for in Vitro Activation of Dormant Follicles for Patients With Primary Ovarian Insufficiency
Stanford Investigator(s)
Eligibility
Inclusion Criteria:
- Married women (18-39) diagnosed with POI/ORS
- With both ovaries present
- With normal uterine cavity
- Healthy and can stand surgery
Exclusion Criteria:
- Any one that does not meet the inclusion criteria
Intervention(s):
procedure: Ovariotomy
procedure: Activate dormant follicles
procedure: Ovary tissue transplantation
procedure: Ovarian superficial cut
Not Recruiting
Contact Information
Stanford University
School of Medicine
300 Pasteur Drive
Stanford,
CA
94305