Study of CRX100 as Monotherapy and in Combination With Pembrolizumab in Patients With Advanced Solid Malignancies


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Trial ID: NCT04282044,37


This clinical study is an open-label, Phase 1, dose-escalation study to determine the safety, tolerability, and efficacy of the drug product produced by Administering CRX100 alone and in combination with Pembrolizumab in advanced solid malignancies. Patients will be screened and evaluated to determine whether or not they meet stated inclusion criteria. Enrolled subjects will undergo leukapheresis to enable the ex vivo generation of CRX100. Patients with non-small cell lung cancer (NSCLC), ovarian cancer, colorectal cancer, hepatocellular carcinoma (HCC), malignant melanoma (excluding uveal melanoma), gastric cancer, triple negative breast cancer, and osteosarcoma. The study will start with monotherapy dose escalation followed by combination cohorts.

Official Title

A Phase 1 Study of the Safety and Efficacy of CRX100 as Monotherapy and in Combination With Pembrolizumab in Advanced Solid Malignancies

Stanford Investigator(s)

Oliver Dorigo, M.D., Ph.D.
Oliver Dorigo, M.D., Ph.D.

Mary Lake Polan Professor


Inclusion Criteria:

Subjects must meet all the following criteria to be enrolled in this study:

   1. Age ≥18 years at the time of consent.

   2. Written informed consent in accordance with national, local, and institutional
   guidelines obtained prior to any study procedures. (Screening assessments performed
   prior to informed consent but within the 28-day screening window are acceptable for
   inclusion purposes).

   3. Subjects must have histologically confirmed diagnosis of one of the following tumors:
   triple negative adenocarcinoma of the breast (human epidermal growth factor receptor 2
   negative, estrogen receptor negative and progesterone receptor negative
   [HER2-/ER-/PR-]), adenocarcinoma of the colon or rectum, hepatocellular carcinoma
   (HCC), osteosarcoma, epithelial ovarian cancer, malignant melanoma, non-small cell
   lung cancer (NSCLC), or gastric cancer. Documentation of the diagnosis with the
   original pathology report, or a recent biopsy, is required.

   4. Subjects must have relapsed disease or refractory disease. Subjects must have
   received, completed, or become intolerant of prior standard of care therapies or are
   not expected to derive any clinical benefit from standard of care therapies.

   5. Subjects with Ovarian cancer must have received at least one prior standard of care
   for their relapsed or refractory disease, which must include a platinum-based regimen.

   6. Subjects agree to provide fresh tumor tissue that has not been previously irradiated.
   If biopsy procedure is not safe to perform, then archival tumor tissue (20 slides or a
   tissue block) can be submitted.

   7. Subjects must have iRECIST evaluable disease using computed tomography (CT) or
   magnetic resonance imaging (MRI) with IV contrast, with at least one measurable target

   8. Subjects must have an Eastern Cooperative Oncology Group (ECOG) performance status of

   9. Subjects are expected to have a life expectancy of at least 12 weeks from the time of

10. Adequate hematologic function at the time of screening, defined as: absolute
   lymphocyte count (ALC) >500 cells/mm3, absolute neutrophil count (ANC) >750 cells/mm3,
   hemoglobin >8 g/dL, and platelet count >50,000 cells/mm3. For subjects enrolling into
   the LDC cohorts, the criteria are defined as: ALC>500 cells/mm3, ANC>1000 cells/mm3,
   hemoglobin>8g/dL, and platelet count>100,000 cells/mm3.

   a. Hemoglobin and platelet count thresholds must be achievable without transfusion of
   red blood cells or platelets, or use of growth factors administered within two weeks.

11. Adequate organ function, defined as:

      1. Renal function: serum creatinine <1.5x institutional upper limit of normal (ULN)
      or calculated creatinine clearance >50 mL/min.

      2. Adequate hepatic function: total bilirubin ≤1.5x institutional ULN; aspartate
      aminotransferase (AST) and alanine aminotransferase (ALT) ≤2.5x institutional
      ULN, unless liver metastases are present, in which case it must be ≤5x ULN;
      International Normalized Ratio (INR) ≤1.5. For subjects with HCC, adequate
      hepatic function is defined as: total bilirubin ≤3x institutional upper limit of
      normal, AST/ALT ≤5x institutional ULN, INR ≤1.7, Child-Turcotte-Pugh score <8.

12. Women of childbearing potential (defined as all subjects physiologically capable of
   becoming pregnant) must have negative serum ß-human chorionic gonadotropin (hCG) or
   urine pregnancy test.

13. Women of childbearing potential must agree to use highly effective methods of
   contraception throughout the study and for six months after the last dose of CRX100.

14. Males who have partners of childbearing potential must agree to use an effective
   barrier contraceptive method throughout the study and for six months after the last
   dose of CRX100.

15. Subjects must be willing and able to comply with all study procedures, requirements,
   and follow-up examinations.

Exclusion Criteria:

Subjects who meet any of the following criteria will be excluded from participation in this

   1. Subjects with new or progressive brain metastasis. Subjects with treated brain
   metastases are eligible if there is no evidence of progression for at least four weeks
   after central nervous system-directed treatment, as ascertained by clinical
   examination and brain imaging (MRI or CT) during the screening period.

   2. Subjects who received any chemotherapy or immunotherapies (non-cell-based therapies)
   or oncolytic virus therapy, radiotherapy, radiosurgery or investigational agents
   within three weeks of enrollment.

   3. Subjects who still are experiencing Grade 2 or higher AEs from prior therapies such as
   surgery, radiation therapy and systemic anti-cancer therapies unless approved by

   4. Subjects who received any type of cell-based therapies within the last 12 weeks from
   the planned apheresis date.

   5. Subjects experiencing any active infections (bacterial, viral, or fungal) for which
   systemic antimicrobials are required. Subjects who need prophylactic anti-viral agents
   that can inhibit the replication of VACV will be excluded from participating.

   6. Subjects must not have history of active or symptomatic autoimmune disease or evidence
   of symptomatic autoimmune pneumonitis, glomerulonephritis, vasculitis, other
   symptomatic autoimmune disease, documented history of autoimmune disease or syndrome
   requiring systemic treatment in the past two years (i.e., with use of disease
   modifying agents, steroids or immunosuppressive agents) Exceptions are permitted for
   vitiligo, type I diabetes mellitus, residual hypothyroidism due to autoimmune
   condition requiring only hormone replacement, psoriasis not requiring systemic
   treatment, conditions not expected to recur in the absence of an external trigger or
   other conditions approved by the medical monitor.

   7. Have a condition requiring systemic treatment with either corticosteroids (>10 mg
   daily prednisone or equivalent) or other immunosuppressive medications within 14 days
   prior to apheresis, and within 14 days prior to infusion. Inhaled, intramuscular
   injection, or topical steroids and adrenal replacement doses (≤10 mg daily prednisone
   equivalents) are permitted. Stable doses of steroids are permitted for subjects with
   pre-treated brain metastases. Short-term (<48 hr) steroid pretreatment for contrast
   allergy for imaging is permitted.

   8. Known human immunodeficiency virus (HIV) infection, active chronic hepatitis B or C,
   life-threatening illnesses unrelated to cancer, or any serious medical or psychiatric
   illness that could, in the Investigator's opinion, interfere with participation in
   this study.

   9. Pregnant or nursing an infant (subject or household contacts).

10. Clinically significant immunodeficiency (e.g., due to underlying illness and/or
   medication) in a subject or household contacts.

11. Have any underlying medical condition (including, but not limited to, ongoing or
   active infection requiring treatment, symptomatic congestive heart failure, unstable
   angina pectoris, or cardiac arrhythmia), psychiatric condition that, in the opinion of
   the Investigator, would compromise study administration as per protocol or compromise
   the assessment of AEs.

12. Have a history of another invasive malignancy, except for the following circumstance:
   individuals with a history of invasive malignancy are eligible if they have been
   disease free and off treatment for at least two years or are deemed by the
   Investigator to be at low risk for recurrence of that malignancy; individuals with the
   following cancers are eligible if diagnosed and treated: carcinoma in situ of the
   breast, oral cavity, or cervix, localized prostate cancer, or basal cell or squamous
   cell carcinoma of the skin. When enrolling a subject with another malignancy, the
   Investigator must discuss the subject with the Medical Monitor.


biological: CRX100 suspension for infusion

combination product: Fludarabine

combination product: Cyclophosphamide


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Contact Information

Stanford University
School of Medicine
300 Pasteur Drive
Stanford, CA 94305
Bela A Shah
(650) 723-0594

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